1. INTENDED USE

The fGIA/TRF Ag Rapid Test is a dual-target fluorescence immunoassay designed for the simultaneous detection and differentiation of:

• Giardia intestinalis (common intestinal parasite)
• Tritrichomonas foetus (a major cause of feline diarrhea)

in feline feces or rectal swab samples. This test assists veterinarians in:

• Rapid diagnosis of chronic diarrhea or large bowel disease
• Differential diagnosis between parasitic infections (Giardia vs. T. foetus)
• Monitoring treatment efficacy post-antiprotozoal therapy

Note: For veterinary point-of-care use. Not for human diagnostics.

2. TEST PRINCIPLE

Utilizing dual-channel fluorescence immunochromatography:

1. Giardia Detection Channel

1. Sandwich Assay:
   • Anti-Giardia monoclonal antibodies (test line) capture Giardia-specific antigens
   • Fluorescent nanoparticle-labeled antibodies form a sandwich complex

2. T. foetus Detection Channel
   2. Competitive Assay:

• T. foetus antigens compete with fluorescent analogs for antibody binding sites
• Negative correlation between pathogen load and fluorescence intensity

3. Detection:
   3. A handheld analyzer quantitatively measures:

• Giardia: ≥85% sensitivity at 50 cysts/μL
• T. foetus: ≥90% sensitivity at 10 organisms/μL

1. Results in 12-15 minutes with automatic interpretation

Key Features:

• Cross-reactivity Free: No interference between two pathogens
• High Throughput: Compatible with batch testing (up to 10 samples/run)
• Feline-Specific: Optimized for feline antigen epitopes

Storage: Keep test cassettes at 2-30°C. Use fresh samples (<2hrs at RT or <72hrs at 4°C).*