1. INTENDED USE

The fGIA Ag Rapid Test is a fluorescence-based immunoassay designed for the qualitative detection of Giardia intestinalis-specific antigens in feline fecal samples. This test provides veterinarians with:

• Rapid diagnosis of active Giardia infection in cats
• Differentiation from other causes of diarrhea (e.g., dietary, viral, or bacterial)
• Monitoring of treatment efficacy and potential reinfections

Note: For veterinary diagnostic use only. Results should be interpreted in conjunction with clinical signs and other diagnostic methods.

2. TEST PRINCIPLE

The test utilizes a sandwich fluorescence immunoassay format:

1. Sample Application: Fecal extract is added to the test device, where Giardia antigens (if present) bind to anti-Giardia monoclonal antibodies conjugated with fluorescent nanoparticles.
2. Lateral Flow: The antigen-antibody complex migrates to the test line containing immobilized anti-Giardia antibodies, forming a fluorescent “sandwich” complex.
3. Detection: A portable fluorescence reader quantifies the signal intensity at 530nm excitation, providing:
   • Qualitative results (Positive/Negative) within 10 minutes
   • High sensitivity (detects as few as 5 cysts per 100μL sample)

Key Features:

• Species-specific: Detects feline Giardia variants (Assemblage F)
• No cross-reactivity: With other common feline parasites (e.g., Toxocara, Coccidia)
• Room temperature storage: Stable for 24 months at 2-30°C

For optimal results:

• Use fresh or properly preserved fecal samples
• Follow recommended sample preparation procedures
• Interpret results within the specified time window