1. INTENDED USE

The CPIV Ag Rapid Test is a fluorescence-based immunoassay designed for the qualitative detection of Canine Parainfluenza Virus (CPIV) antigen in canine nasal swabs, conjunctival swabs, or tracheal wash samples. This test assists veterinarians in:

• Rapid diagnosis of CPIV infection in dogs with respiratory symptoms (e.g., coughing, nasal discharge, or kennel cough syndrome).
• Differentiation from other canine respiratory pathogens (e.g., Canine Distemper Virus, Bordetella bronchiseptica).
• Infection control in kennels, shelters, or multi-dog environments.

Note: For veterinary point-of-care use only. Results should be interpreted in conjunction with clinical signs and other diagnostic tests.

2. TEST PRINCIPLE

The test utilizes a sandwich fluorescence immunoassay format:

1. Capture Antibody: Monoclonal anti-CPIV antibodies immobilized on the test strip bind CPIV antigen present in the sample.
2. Fluorescent Detection: A second antibody conjugated with fluorescent nanoparticles binds to the captured CPIV antigen, forming an antibody-antigen-antibody complex.
3. Signal Measurement: A handheld fluorescence reader quantifies the signal intensity, providing qualitative results (Positive/Negative) within 10 minutes.

Key Features:

• High Sensitivity: Detects low levels of CPIV antigen (LoD: 1.0×10³ TCID50/mL).
• No Cross-Reactivity: Specific to CPIV (no interference from other common canine respiratory pathogens).
• User-Friendly: Minimal sample preparation required; compatible with fresh clinical samples.

For accurate results, collect samples during acute infection and avoid excessive mucus or blood contamination.