1. INTENDED USE

The CCV Ag Rapid Test is a fluorescence-based immunoassay designed for the qualitative detection of Canine Coronavirus Antigen in canine fecal samples. This test provides veterinarians and pet owners with:

• Early screening for CCV infection in dogs with diarrhea or vomiting
• Differential diagnosis of viral enteritis (alongside CPV, CDV testing)
• Ken4nel/cattery management to identify and isolate infected animals

Note: For veterinary diagnostic use only. Clinical symptoms should be correlated with test results.

2. TEST PRINCIPLE

The test utilizes a sandwich fluorescence immunoassay methodology:

1. Sample Application: Fecal suspension is added to the test device, releasing CCV antigens
2. Antigen-Antibody Binding:
   • CCV antigens bind to anti-CCV monoclonal antibodies conjugated with fluorescent nanoparticles
   • The complex migrates to react with immobilized anti-CCV antibodies at the test line
3. Signal Generation:
   • Accumulation of fluorescent complexes produces a measurable signal
   • The portable reader detects fluorescence intensity at 620nm within 5-8 minutes

Key Features:

• High Sensitivity: Detects as low as 1×10³ TCID50/mL
• No Prozone Effect: Accurate across high/low viral loads
• Stable Results: Fluorescent signal remains readable for 48 hours

*Best performance with fresh fecal samples (<24h). Store test devices at 2-30°C.*