1. INTENDED USE

The Canine Triple Antibody Rapid Test is a multiplex fluorescence immunoassay designed for simultaneous detection and semi-quantitative measurement of protective antibodies against three critical canine diseases:

• Infectious Canine Hepatitis (ICH)
• Canine Parvovirus (CPV)
• Canine Distemper Virus (CDV)

This test assists veterinarians in:

• Vaccination status assessment (evaluating antibody levels post-vaccination)
• Disease management (identifying susceptible animals in outbreak situations)
• Breeding colony health monitoring
• Pre-surgical/travel health screening

Note: For veterinary use only. Does not differentiate between vaccine-induced and natural infection antibodies.

2. TEST PRINCIPLE

The test utilizes multiplex fluorescence immunochromatography:

1. Triplex Test Strip: Contains three separate detection lines coated with:
   • ICH virus-like particles (VLPs)
   • CPV VP2 antigen
   • CDV nucleocapsid protein
2. Fluorescent Conjugate: Serum antibodies bind to:
   • Disease-specific antigens on the strip
   • Fluorescent anti-canine IgG conjugates (quantitative detection)
3. Reading Method:
   • A portable reader measures line-specific fluorescence at:
     • 680nm (ICH)
     • 720nm (CPV)
     • 610nm (CDV)
   • Results interpreted as:
     • Negative (<50% reference level)
     • Low-positive (50-80%)
     • Protective (>80%)

Key Features:

• 15-minute procedure with 5μL serum/plasma
• Cross-reactivity <0.1% between targets
• Stable at 2-30°C (no cold chain required)

Includes internal control line. Refer to species-specific cutoff values in the package insert.