INTENDED USE
The Yellow Fever Virus NS1 Rapid Test is a lateral flow chromatographic immunoassay for the qualitative detection of Yellow Fever Virus antigen in human serum ,plasma or whole blood. It is intended to be used as a screening test and as an aid in the diagnosis of infection with Yellow Fever Virus. Any reactive specimen with the Yellow Fever Virus Ag Rapid Test must be confirmed with alternative testing method(s) and clinical findings.

SUMMARY AND EXPLANATION THE TEST
IgG/IgM antibody is produced one week after onset, so the detection of IgG/IgM is of great significance for the early diagnosis of Yellow Fever Virus virus.Yellow Fever Virus is diagnosed based on serological analysis and viral isolation in mice or tissue culture. An IgG/IgM immunoassay is the most practical lab test method. The Yellow Fever Virus IgM/IgG Rapid Test utilizes recombinant antigens derived from its structure protein, it detects IgM/IgG anti-Yellow Fever Virus in patient serum or plasma within 15 minutes. The test can be performed by untrained or minimally skilled personnel, without cumbersome laboratory equipment.

PRINCIPLE
The Yellow Fever Virus NS1 Rapid Test is a lateral flow chromatographic immunoassay. The test cassette consists of: 1) a burgundy colored conjugate pad containing mouse anti-Yellow Fever Virus NS1 antibody conjugated with colloid gold (Yellow Fever Virus Ab conjugates), 2) a nitrocellulose membrane strip containing a test band (T band) and a control band (C band). The T band is pre-coated with rabbit anti-Yellow Fever Virus NS1 antibody, and the C band is pre-coated with goat anti-mouse IgG antibody. The antibodies to Yellow Fever Virus antigen recognize the antigens from all the four serotypes of the Yellow Fever Virus virus.
When an adequate volume of test specimen is dispensed into the sample well of the cassette, the specimen migrates by capillary action across the test cassette.Yellow Fever Virus NS1 Ag if present in the specimen will bind to the Yellow Fever Virus Ab conjugates. The immunocomplex is then captured on the membrane by the pre-coated rabbit anti-Yellow Fever Virus NS1 antibody, forming a burgundy colored T band, indicating a Yellow Fever Virus Ag positive test result. Absence of the T band suggests a negative result. The test contains an internal control (C band) which should exhibit a burgundy colored band of the immunocomplex of goat anti-mouse IgG/mouse IgG-gold conjugate regardless of the presence of colored T band. Otherwise, the test result is invalid and the specimen must be retested with another device.