INTENDED USE

Transmissible gastroenteritis (TGE) in swine is one of the most significant diarrhea-causing diseases in young pigs and is caused by transmissible gastroenteritis virus (TGEV). TGEV belongs to the genus Alphacoronavirus, family Coronaviridae and contains a single-stranded, positive-sense RNA genome of approximately 28.5 kb in length. The enteropathogenic coronavirus causes severe diarrhea, vomiting, and dehydration, with mortality rates of greater than 95% in piglets less than 2 weeks old. TGEV infection damages the small intestine, impairs immune functions, and increases pathogenic bacterial loading, all of which may facilitate secondary infections by other pathogens. The infection was usually mild to moderate and focal in the pigs without clinical signs of the disease and more severe and extensive in the pigs with clinical signs of the disease variable in severity.
TGEV spreads rapidly by aerosol or contact exposure and is infected through the oral or nasal route. The surviving pigs continue to shed the virus from their stool or nasal secretions for 2 to 8 weeks. Some pigs excrete the virus intermittently for up to 18 months.

Principle

TGEV Ag Test Kit is a lateral flow chromatographic immunoassay for the qualitative detection of TGEV antigens in porcine feces. This kit shows two letters which are the test (T) line and the control (C) line on the surface of the device. If the TGEV antigen exists in the sample, it binds to the gold-conjugated TGEV monoclonal antibody. The antigen-antibody complex moves through the membrane by capillary force and responds to the TGEV monoclonal antibody on the test line, resulting in a red line. The control line indicates that the test is performed correctly and should appear when the test is complete.
The highly selective and sensitive two monoclonal antibodies to TGEV are used as capture and detector in the kit. The TGEV Ag Test Kit can detect TGEV antigen in porcine feces with high accuracy.