Transferrin Antigen
Catalog No. | STFRAGF |
---|---|
Specimen | Human Feces |
Dimensions | Height: 60mm, Length: 300mm, Height: 84mm, Length: 300mm |
Intended Use
The Transferrin Antigen Rapid Test Kit is a rapid chromatographic immunoassay for the qualitative detection of transferrin in human Fecal Specimen.
Product Description
INTENDED USE
The Transferrin Antigen Rapid Test Kit is a rapid chromatographic immunoassay for the qualitative detection of transferrin in human Fecal Specimen.
INTRODUCTION
Many diseases can cause hidden blood in the stool. In the early stages, gastrointestinal problems such as colon cancer, ulcers, polyps, colitis, diverticulitis, and fissures may not show any visible symptoms, only occult blood. Traditional guaiac-based method lacks sensitivity and specificity, and has diet-restrictions prior to testing.
Tf is a type of β1 globulin, with a molecular weight of 77 KD, accounts for about 0.3% to 0.5% of the total plasma protein. It is mainly synthesized in liver, and the main function of the protein is to transport extracellular iron into cells through membrane receptor-mediated endocytosis. 3 As in the case of transferrin, serum Tf may be leaked into gastrointestinal tract during the course of bleeding. However, compared with transferrin, Tf is more stable.
Human Transferrin Antigen Rapid Test Kit (Colloidal Gold)is a rapid test to qualitatively detect low levels of Transferrin of fecal occult blood in feces. The test uses double antibody sandwich assay to selectively detect as low as 40ng/mL transferrin of human occult blood in feces. In addition, unlike the guaiac assays, the accuracy of the test is not affected by the diet of the patients.
PRINCIPLE
Transferrin Antigen Rapid Test Kit is a qualitative, lateral flow immunoassay for the detection of human occult blood in feces. The membrane is pre-coated with anti-transferrin antibody on the test line region of the strip. During testing, the specimen reacts with the particle coated with anti-transferrin antibody. The mixture migrates upward on the membrane chromatographically by capillary action to react with anti-transferrin antibody on the membrane and generate a colored line. The presence of this colored line in the test region indicates a positive result, while its absence indicates a negative result. To serve as a procedural control, a colored line will always appear in the control line region indicating that the proper volume of specimen has been added and membrane wicking has occurred.