INTRODUCTION

Influenza is a highly contagious, acute, viral infection of the respiratory tract. The causative agents of the disease are immunologically diverse, single-strand RNA viruses known as influenza viruses. There are three types of influenza viruses: A, B, and C. Type A viruses are the most prevalent and are associated with most serious epidemics. Type B viruses produce a disease that is generally milder than that caused by type A. Type C viruses have never been associated with a large epidemic of human disease. Both type A and B viruses can circulate simultaneously, but usually one type is dominant during a given season. Influenza antigens may be detected in clinical specimens by immunoassay.
The novel coronaviruses belong to the βgenus. COVID-19 is an acuterespiratory infectious disease. People are generally susceptible. Currently, the patients infected by the novel coronavirus are the main source of infection; asymptomatic infected people can also be an infectious source. Based on the current epidemiological investigation, the incubation period is 1 to 14 days, mostly 3 to 7 days. The main manifestations include fever, fatigue and dry cough. Nasal congestion, runny nose, sore throat, myalgia and diarrhea are found in a few cases.
SARS-COV-2 Antigen/Influenza A+B Antigen Rapid Test Kit (Nasal Swab Test) is a lateral-flow immunoassay using highly sensitive monoclonal antibodies that are specific for influenza types A and B and SARS-CoV-2 antigens. The test is specific to influenza types A and B and SARS-CoV-2 antigens with no known cross- reactivity to normal flora or other known respiratory pathogens.

PRINCIPLE

SARS-COV-2 Antigen/Influenza A+B Antigen Rapid Test Kit (Nasal Swab Test) detects influenza A and B viral antigens and SARS-CoV-2 Antigen through visual interpretation of color development on the strip. Anti-influenza A and B antibodies , Anti-SARS-CoV-2 antibodies and Goat anti-Rabbit IgG Antibodies are immobilized on the test region A, B, N and C of the membrane respectively. During testing, the extracted specimen reacts with anti-influenza A, B, SARS-CoV-2 antibodies and rabbit IgG Antibodies conjugated to colored particles and precoated onto the sample pad of the test. The mixture then migrates through the membrane by capillary action and interacts with reagents on the membrane.

If there is sufficient influenza A and B viral antigens or SARS-CoV-2 antigen in the specimen, colored band(s) will form at the according test region of the membrane. The presence of a colored band in the A and/or B and/or N region indicates a positive result for the particular viral antigens, while its absence indicates a negative result.The appearance of a colored band at the control region serves as a procedural control, indicating that the proper volume of specimen has been added and membrane wicking has occurred.