INTRODUCTION

Respiratory Syncytial Virus(RSV) is the most common cause of bronchiolitis and pneumonia among infants and children under 1 year of age.IIIness begins most frequently with fever,runny nose,cough and sometimes wheezing. Severe lower respiratory tract disease may occur at any age,especially among the elderly or among those with compromised cardiac, pulmonary or immune systems.RSV is spread from respiratory secretions through close contact with infected persons or contact with contaminated surfaced or objects.

PRINCIPLE

The Respiratory Syncytial Virus(RSV) Antigen Rapid Test Kit is a lateral flow chromatographic immunoassay. The test cassette consists of: 1) a burgundy colored conjugate pad containing recombinant antigen conjugated with colloid gold (monoclonal mouse anti Respiratory Syncytial Virus(RSV) antibody conjugates) and rabbit IgG-gold conjugates, 2) a nitrocellulose membrane strip containing test band (T bands) and a control band (C band). The T band is pre-coated with monoclonal mouse anti- Respiratory Syncytial Virus(RSV) antibody for the detection of Respiratory Syncytial Virus(RSV) glycoprotein F antigen, and the C band is pre-coated with goat anti rabbit IgG. When an adequate volume of test specimen is dispensed into the sample well of the test cassette, the specimen migrates by capillary action across the cassette. Respiratory Syncytial Virus(RSV) if present in the specimen will bind to the monoclonal mouse anti-Respiratory Syncytial Virus(RSV) antibody conjugates. The immunocomplex is then captured on the membrane by the pre-coated mouse anti-Respiratory Syncytial Virus(RSV) antibody, forming a burgundy colored T band, indicating a Respiratory Syncytial Virus(RSV) antigen positive test result.

Absence of test band (T) suggests a negative result. The test contains an internal control (C band) which should exhibit a burgundy colored band of the immunocomplex of goat anti rabbit IgG/rabbit IgG-gold conjugate regardless of the color development on any of the test bands. Otherwise, the test result is invalid, and the specimen must be retested with another device.