INTENDED USE

The Human Rhinovirus Antigen Rapid Test Kit is a lateral flow chromatographic immunoassay for the qualitative detection of Novel coronavirus in human swab (oropharyngeal swab, nasopharyngeal swabs and Anterior nasal swab). It is suitable for the auxiliary diagnosis of Rhinovirus infection.

PRINCIPLE

The Human Rhinovirus Antigen Rapid Test Kit is a lateral flow chromatographic immunoassay. The test cassette consists of: 1) a burgundy colored conjugate pad containing recombinant antigen conjugated with colloid gold (monoclonal mouse anti Human Rhinovirus antibody conjugates) and rabbit IgG-gold conjugates, 2) a nitrocellulose membrane strip containing test band (T bands) and a control band (C band). The T band is pre-coated with monoclonal mouse anti- Human Rhinovirus antibody for the detection of Human Rhinovirus antigen, and the C band is pre-coated with goat anti rabbit IgG. When an adequate volume of test specimen is dispensed into the sample well of the test cassette, the specimen migrates by capillary action across the cassette. Human Rhinovirus if present in the specimen will bind to the monoclonal mouse anti-Human Rhinovirus antibody conjugates. The immunocomplex is then captured on the membrane by the pre-coated mouse anti-Human Rhinovirus antibody, forming a burgundy colored T band, indicating a Human Rhinovirus antigen positive test result. Absence of test band (T) suggests a negative result. The test contains an internal control (C band) which should exhibit a burgundy colored band of the immunocomplex of goat anti rabbit IgG/rabbit IgG-gold conjugate regardless of the color development on any of the test bands. Otherwise, the test result is invalid, and the specimen must be retested with another device.