INTENDED USE

Toxoplasma gondii is the protozoan parasitic agent of toxoplasmosis, a zoonotic disease of significant medical and veterinary importance worldwide. T. gondii, a member of the family Sarcocystidae in the phylum Apicomplexa, developed the ability to infect almost any cell type of mammals and birds.
Domesticated and wild cats are the only known definitive hosts of T. gondii and are the main reservoirs of infection. The clinical signs of toxoplasmosis in cats are generally fever, anorexia, or dyspnea, and are specifically neural, respiratory, cutaneous, or ocular involvement. Fecal T. gondii oocysts are shed in large numbers by acutely infected cats once for approximately two weeks, except in cases of feline immune-suppression, such as co-infection with feline immunodeficiency virus or feline leukemia virus, which can result in secondary shedding. The disease transmissible oocysts can survive in the environment for several months to more than 1 year, are remarkably resistant to disinfectants, freezing, and drying but are killed by heating to 70°C for 10 minutes.
Toxoplasmosis can cause severe ocular and neurological disease in humans. People become infected through the accidental consumption of feline fecal material, through food or water with fecal contamination, through the consumption of undercooked meat containing infective T. gondii cysts, through transplantation, or vertically from mother to fetus.

PRINCIPLE

The Toxoplasma Ab Test Kit is a lateral flow chromatographic immunoassay for the qualitative detection of Toxoplasma antibodies in feline blood. This kit shows two letters which are the test (T) line and the control (C) line on the surface of the device. If the Toxoplasma antibody exists in the sample, it binds to the gold-conjugated recombinant Toxoplasma antigen. The antibody-antigen complex moves through the membrane by capillary force and responds to the Toxoplasma antigen on the test line, resulting in a red line. The control line indicates that the test is performed correctly and should appear when the test is complete.
The high-quality recombinant T. gondii antigen [surface antigen 1 (SAG1: p30) + dense granule protein (GRA1: p24)] is used as capture and detector in the kit. The Toxoplasma Ab Test Kit can detect Toxoplasma antibodies in feline blood with high accuracy.