Canine parvovirus antibody (CPV Ab)
| Catalog No. | SCPVABS |
|---|---|
| Specimen | Serum/Plasma |
| Dimensions | Height: 60mm, Length: 300mm, Height: 84mm, Length: 300mm |
Intended Use
Canine parvovirus (CPV) is the major viral disease in dogs in the world, which cause gastroenteritits resulting to severe enteritis and lymphopenia having high motality in dogs. CPV antibody can give seroprotection against its infection in dogs. Therefore, CPV antibody titers are very important in the vaccination programs and health prediction in dogs.
Product Description
INTENDED USE
CPV Ab is a chromatographic immunoassay kit for rapid and quantitative detection of canine parvovirus antibody (CPV Ab) from whole blood, serum or plasma specimen of dog.
SUMMARY
A nitrocellulose membrane is immobilized with anti-canine IgG monoclonal antibody and anti-NUS antibody in the test line and control line respectively. CPV Ab for test line and NUS for control line are conjugated to colloidal gold particles. This conjugate is placed on a polyester pad as a conjugate pad. This test is aimed to the detection of CPV antibody either in whole blood, serum or plasma. When the device is loaded with assay solution containing specimens, the anti-CPV antibodies of the sample firstly react with the CPV antigens of the antigen pad, and secondly react with the anti-CPV gold conjugate of the conjugate pad. Then, the complex comes into contact with the anti-canine IgG that it adsorbed onto the nitrocellulose. The result is visible within 10 minutes in the form of a purple line on the test line (T) region of the device. The solution continues to migrate to encounter anti-NUS, thereby producing a second purple line on the control line (C) area.
PRINCIPLE
The CPV Ag Test Kit is a lateral flow chromatographic immunoassay for the qualitative detection of CPV antigens in canine feces. This kit shows two letters which are the test (T) line and the control (C) line on the surface of the device. If the CPV antigen exists in the sample, it binds to the gold-conjugated CPV antibody. The antigen-antibody complex moves through the membrane by capillary force and responds to the CPV antibody on the test line, resulting in a red line. The control line indicates that the test is performed correctly and should appear when the test is complete.
The highly selective and sensitive monoclonal antibody to CPV is used as a capture and detector in the kit. The CPV Ag Test Kit can detect CPV antigen in canine feces with high accuracy.
