INTENDED USE

It can be used to detect enterotoxin in fecal specimens from persons suspected of having C. perfringens disease. The test is to be used as an aid in the diagnosis of C. perfringens enterotoxin-associated disease and results should be considered in conjunction with the patient history.

Pinciple

The Clostridium perfringens Enterotoxin Test uses two antibodies to C. perfringens enterotoxin. The microassay wells supplied with the kit contain immobilized polyclonal antibody against C. perfringens enterotoxin. The Conjugate consists of polyclonal antibody conjugated to horseradish peroxidase. In the assay, an aliquot of a fecal specimen is emulsified in the Diluent and the diluted specimen is then transferred to the microassay well. If enterotoxin is present in the specimen, it will bind to the immobilized polyclonal antibody and conjugate during the 2-hour incubation phase. Any unbound material is removed during the washing steps. Following the addition of Substrate, a color is detected due to the enzyme-antibody-antigen complexes that form in the presence of enterotoxin.