INTENDED USE

Canine influenza viruses (CIV), belong to the species Influenza A virus, genus Alphainfluenzavirus and family Orthomyxoviridae, and cause a highly contagious respiratory disease. Two major influenza viruses have been acquired in dogs since 1999, the subtype H3N8 came from horses and the subtype H3N2 came from birds. The subtype H5N1 and H1N1 have also been isolated from dog flu.

SUMMARY

The most common symptom of 80% of exposed dogs is a mild upper respiratory disease with 1 to 3 weeks persistent cough. Other possible clinical signs include ocular and nasal discharge, sneezing, fever, lethargy, and anorexia. Some dogs become severely ill, with high fever, pneumonia, and secondary bacterial infection. The mortality rate is lower than 10%.
In mammals, influenza viruses are usually transmitted in droplets and aerosols created by coughing and sneezing, and by contact with nasal discharges, either directly or indirectly. CIV are found in respiratory secretions, like the typical mammalian influenza viruses.
Influenza is one of the most important zoonoses. There are no reports of human infections with CIV to date, although such infections are theoretically possible.

PRINCIPLE

CIV Ag Test Kit is a lateral flow chromatographic immunoassay for the qualitative detection of CIV antigens in canine pharyngeal or nasal secretions. This kit shows two letters which are the test (T) line and the control (C) line on the surface of the device. If the CIV antigen exists in the sample, it binds to the cellulose nanobeads (CNB)-conjugated CIV antibody. The antigen-antibody complex moves through the membrane by capillary force and responds to the CIV antibody on the test line, resulting in a red line. The control line indicates that the test is performed correctly and should appear when the test is complete.
The highly selective and sensitive two monoclonal antibodies to CIV are used as capture and detector in the kit. CIV Ag Test Kit can detect CIV antigens in canine nasal or pharyngeal secretions with high accuracy.