INTENDED USE

Canine distemper is highly contagious and fetal disease of dogs with a worldwide distribution. Canine distemper virus (CDV, the species Canine morbillivirus), an enveloped, negative-sense, single-stranded RNA virus, is a member of the genus Morbillivirus in the family Paramyxoviridae.

SUMMARY

CDV infects different cell types, including epithelial, mesenchymal, neuroendocrine and hematopoietic cells of various organs and tissues. A diphasic fever is a characteristic feature of the disease, occurring 7 or 8 days after infection, that drops rapidly and again climbs by day 11 or 12. Clinical signs of distemper are often unapparent or initially mild during this time, and the disease is characterized by mucopurulent oculonasal discharges, conjunctivitis, respiratory distress, anorexia, vomiting, diarrhea and dehydration, and cutaneous rash. Additional clinical signs that may occur are localized twitching, ascending paresis/paralysis, and/or convulsions. Hyperkeratosis of the foot pads and nose also may occur. Dogs that recover from acute disease with persistent infection may shed virus in urine and through the skin on the foot pads.
Domestic dogs are the main host for CDV and could also be considered as a reservoir for other mammals. Due to its high morbidity and mortality rates and broad host range, CDV is not only a well-known pathogen of domestic dogs but also one of the most important pathogens of mammals with the ability to jump the species barrier and perhaps even infect humans.

PRINCIPLE

The CDV Ag Test Kit is a lateral flow chromatographic immunoassay for the qualitative detection of CDV antigens in canine eye discharges. This kit shows two letters which are the test (T) line and the control (C) line on the surface of the device. If the CDV antigen exists in the sample, it binds to the gold-conjugated anti-CDV antibody. The antigen-antibody complex moves through the membrane by capillary force and responds to the anti-CDV antibody on the test line, resulting in a red line. The control line indicates that the test is performed correctly and should appear when the test is complete.
The highly selective and sensitive monoclonal antibody to CDV is used as a capture and detector in the kit. The CDV Ag Test Kit can detect CDV in canine eye discharge (conjunctiva) with high accuracy.