INTENDED USE

African swine fever virus (ASFV), a large double stranded DNA virus, genus Asfivirus in the only member of the Asfarviridae family, is known as not infectious to humans and not directly affect public health.

African swine fever (ASF) is a lethal hemorrhagic fever of pigs with high mortality and morbidity rates approaching 100 percent. ASF was first reported in Southeast African countries, including Kenya, in the 1920s. The spread of ASF outside Africa where was first described is persistent in Europe, the Russian Federation, China, Mongolia and Vietnam. It also has posed a serious threat of animal health, pig production and global food security. Since its introduction to and dramatic spread in 2018 in China, which has half of the world’s pig population, current epidemio-logical situation keeps impact on the pig industry.
Apparent symptoms of disease are eruptions, in which the skin turns pinkish and nearly purple marks on the skin of the extremities, ears, chest, abdomen and perineum. Moreover, abortion can frequently occur in gestating females and be sometimes the first clinical sign of an outbreak.
At present, there are no treatment or vaccines available to prevent ASF so the control strategy mainly relies on strict administration of sanitary measures and slaughtering of infected and exposed animals. Therefore, rapid and accurate diagnosis of the infections is essential for prevention, control, and extermination of the disease in affected various countries. Point-of-care rapid and sensitive detection of ASFV is key to the timely implementation of control.

Pinciple

The ASFV Ag Test Kit is a lateral flow chromatographic immunoassay for the qualitative detection of ASFV in porcine blood. This kit shows two letters which are the test (T) line and the control (C) line on the surface of the device. If the ASFV antigen exists in the sample, it binds to the gold-conjugated anti-ASFV antibody. The antigen-antibody complex moves through the membrane by capillary force and responds to the secondary anti-ASFV antibody on the test line, resulting in a red line. The control line indicates that the test is performed correctly and should appear when the test is complete. Two different monoclonal antibodies to the conservative phosphoprotein p30 protein8 of ASFV are used as a capture and detector in the kit. The ASFV Ag Test Kit can detect ASFV in porcine blood with high accuracy.