INTRODUCTION

Adenovirus most commonly cause respiratory illness,however,depending on the infecting serotype,they may also cause various other illnesses,such as gastroenteritis,conjunctivitis,cystitis and rash illness.Symptoms of respiratory illness caused by Adenovirus infection range from the common cold syndrome to pneumonia,croup and bronchitis. Patients with compromised immune systems are especially susceptible to severe complications of Adenovirus is transmitted by direct contact,fecal-oral transmission and occasionally waterborne transmission.Some types are capable of establishing persistent asymptomatic infections in tonsils,adenoids,and intestines of infected hosts and shedding can occur for months or years.

PRINCIPLE

The Adenovirus Antigen Rapid Test Kit is a lateral flow chromatographic immunoassay. The test cassette consists of: 1) a burgundy colored conjugate pad containing recombinant antigen conjugated with colloid gold (monoclonal mouse anti-Adenovirus antibody conjugates) and rabbit IgG-gold conjugates, 2) a nitrocellulose membrane strip containing test band (T bands) and a control band (C band). The T band is pre-coated with monoclonal mouse anti- Adenovirus antibody for the detection of Adenovirus antigen, and the C band is pre-coated with goat anti rabbit IgG. When an adequate volume of test specimen is dispensed into the sample well of the test cassette, the specimen migrates by capillary action across the cassette. Adenovirus if present in the specimen will bind to the monoclonal mouse anti-Adenovirus antibody conjugates. The immunocomplex is then captured on the membrane by the pre-coated mouse anti-Adenovirus antibody, forming a burgundy colored T band, indicating a Adenovirus antigen positive test result.
Absence of test band (T) suggests a negative result. The test contains an internal control (C band) which should exhibit a burgundy colored band of the immunocomplex of goat anti rabbit IgG/rabbit IgG-gold conjugate regardless of the color development on any of the test bands. Otherwise, the test result is invalid, and the specimen must be retested with another device.