1. INTENDED USE

The Veterinary TOXO Ag Rapid Test is a fluorescence-based immunoassay designed for the qualitative detection of Toxoplasma gondii antigens in feline serum, plasma, or whole blood. This test enables:

• Early diagnosis of acute toxoplasmosis in cats, the definitive host of T. gondii
• Differentiation between active infection and past exposure (antigen detection indicates active replication)
• Monitoring treatment efficacy in infected animals
• Screening of high-risk populations (e.g., immunosuppressed cats or multi-cat environments)

Note: For veterinary use only. Should be interpreted in conjunction with clinical signs and other diagnostic methods.

2. TEST PRINCIPLE

The test utilizes a sandwich fluorescence immunoassay format with the following steps:

1. Capture Phase:
   • Monoclonal anti-TOXO antibodies are immobilized on the test strip
   • TOXO antigens in the sample bind to these antibodies
2. Detection Phase:
   • Fluorescent nanoparticle-conjugated anti-TOXO antibodies form complexes with captured antigens
   • Unbound materials are washed away
3. Signal Measurement:
   • A portable fluorescence reader quantifies the signal intensity
   • Results are available in 15 minutes with a detection limit of 0.1 ng/mL

Key Features:
✔ High sensitivity – detects circulating antigen during early infection
✔ No prozone effect – accurate across wide antigen concentrations
✔ Species-specific – optimized for feline samples
✔ Stable signal – fluorescence detection minimizes interference from sample color or turbidity

Storage: Store test kits at 2-8°C. Bring to room temperature before use.
Sample Types: Serum (recommended), plasma, or whole blood (EDTA/heparin)
Interference: Hemolyzed or lipemic samples may affect results