1. INTENDED USE

The Veterinary T4 Rapid Test is a fluorescence-based immunoassay designed for the quantitative measurement of Thyroxine (T4) in canine and feline serum or plasma. This test provides veterinarians with a rapid and accurate tool to:

• Diagnose hypothyroidism in dogs and hyperthyroidism in cats.
• Monitor thyroid hormone replacement therapy (e.g., response to levothyroxine treatment).
• Evaluate overall thyroid function in routine health screenings or pre-surgical assessments.

Note: For veterinary diagnostic use only. Not for human testing.

2. TEST PRINCIPLE

The test employs a competitive fluorescence immunoassay method:

1. Competitive Binding: Endogenous T4 in the sample competes with a fluorescently labeled T4 analog for binding sites on anti-T4 antibodies immobilized on the test strip.
2. Signal Detection: The fluorescence intensity is inversely proportional to the T4 concentration in the sample (higher T4 = lower fluorescence).
3. Quantitative Results: A portable fluorescence reader measures the signal and calculates T4 levels within 8–12 minutes, with a detection range of 5–120 nmol/L.

Key Advantages:

• High Specificity: Minimal cross-reactivity with T3 or other thyroid metabolites.
• Wide Dynamic Range: Covers clinically relevant T4 levels for both dogs and cats.
• Stable Fluorescence Signal: Reduces variability compared to traditional ELISA methods.

For optimal performance, use fresh or properly stored serum/plasma samples and follow manufacturer instructions.