1. INTENDED USE

The Veterinary Brucella Ab Rapid Test is a fluorescence-based immunoassay designed for qualitative/semi-quantitative detection of Brucella-specific antibodies in serum, plasma, or whole blood samples from livestock (cattle, sheep, goats, pigs) and companion animals (dogs). This test assists in:

• Screening Brucellosis infections (zoonotic risk assessment)
• Epidemiological surveillance in herds/flocks
• Differentiating vaccinated vs. infected animals (when used with complementary tests)

Note: For veterinary use only. Confirm positive results with reference methods (e.g., PCR, bacterial culture).

2. TEST PRINCIPLE

The test utilizes an indirect fluorescence immunoassay format:

1. Antigen Coating: Recombinant Brucella LPS/OMP antigens are immobilized on the test strip.
2. Antibody Binding: Brucella-specific antibodies (IgG/IgM) in samples bind to the antigens.
3. Signal Generation: Fluorescent anti-species conjugate (e.g., anti-bovine IgG) binds to captured antibodies.
4. Detection: A fluorescence reader quantifies signal intensity at 530nm excitation/580nm emission, providing:
   • Qualitative: Positive/Negative cutoff (≥15 RU)
   • Semi-quantitative: Antibody titer estimation (RU values)

Key Features:

• Multi-species Adaptability: Adjustable conjugates for bovine/canine/ovine applications
• High Sensitivity: Detects 0.8 IU/mL anti-Brucella antibodies (vs. 2 IU/mL in ELISA)
• No Prozone Effect: Stable fluorescence even at high antibody titers

*Store at 2-8°C. Avoid hemolyzed/lipemic samples for optimal accuracy.*