1. INTENDED USE

The TRF Ag Rapid Test is a fluorescence-based immunoassay designed for the qualitative detection of Tritrichomonas foetus antigen in feline fecal samples. This test enables veterinarians to:

• Diagnose Tritrichomonas foetus infection, a common cause of chronic large-bowel diarrhea in cats
• Screen asymptomatic carrier cats in multi-cat households or catteries
• Monitor treatment efficacy for infected cats receiving antiprotozoal therapy

Note: For veterinary diagnostic use only. Results should be interpreted in conjunction with clinical signs and other diagnostic methods.

2. TEST PRINCIPLE

The test utilizes an immunochromatographic fluorescence assay format:

1. Sample Application: Fecal extract is applied to the test device, where TRF antigens (if present) bind to fluorescent nanoparticle-conjugated anti-TRF antibodies
2. Lateral Flow: The antigen-antibody complexes migrate along the strip and are captured by immobilized anti-TRF antibodies at the test line
3. Fluorescence Detection: A specialized reader quantifies the fluorescence signal at the test line, with results available in 8-10 minutes

Key Features:

• High Sensitivity (92-95%): Detects low levels of TRF antigen, superior to direct microscopy
• Excellent Specificity (98-99%): No cross-reactivity with Giardia or other enteric parasites
• Stable Results: Fluorescent signal remains stable for documentation purposes
• Easy Interpretation: Clear positive/negative results via automated reader output

For optimal performance, use fresh fecal samples (preferably diarrheic) and follow the manufacturer’s sample preparation protocol exactly.