The FPV Ag Rapid Test is a fluorescence-based immunoassay designed for the qualitative detection of Feline Panleukopenia Virus (FPV) antigen in feline feces, vomitus, or serum. This test enables:

• Early diagnosis of FPV infection (feline distemper) with high sensitivity
• Rapid screening in multi-cat environments (shelters, catteries)
• Differentiation from other feline gastrointestinal diseases
• Monitoring of viral shedding during treatment

Note: For veterinary point-of-care use. Confirm positive results with PCR if needed.

2. TEST PRINCIPLE

The test utilizes a sandwich fluorescence immunoassay format:

1. Capture Phase: FPV antigens in samples bind to anti-FPV monoclonal antibodies immobilized on the test membrane
2. Detection Phase: Fluorescent nanoparticle-conjugated antibodies form complexes with captured antigens
3. Signal Amplification: The fluorescence reader detects emitted light at 620nm, with intensity proportional to antigen concentration

Key Features:

• Ultra-Sensitive: Detects as few as 10³ viral particles/gram feces
• Rapid: Provides results in 8-10 minutes
• Cross-Reactivity Free: No interference with canine parvovirus or other feline pathogens
• Stable: Fluorescent signal remains readable for 48 hours post-test

Specimen Collection Tips:

• Optimal detection window: 3-5 days post-infection
• Use fresh fecal samples (<24h old) for best results
• Liquid samples require centrifugation before testing

This test complements but does not replace vaccination status evaluation. Clinical correlation required.