1. INTENDED USE

The FPV Ab Rapid Test is a fluorescence-based immunoassay designed for the qualitative and semi-quantitative detection of antibodies against Feline Panleukopenia Virus (FPV) in feline serum, plasma, or whole blood. This test assists veterinarians in:

• Evaluating vaccination status by measuring protective antibody levels post-vaccination.
• Assessing immune protection in cats prior to high-risk exposures (e.g., catteries, shelters).
• Aiding diagnosis of FPV infection when paired with antigen testing (antibody rise supports recent infection).

Note: For veterinary use only. Results should be interpreted in conjunction with clinical signs and history.

2. TEST PRINCIPLE

The test utilizes an indirect fluorescence immunoassay format:

1. Antigen Coating: Recombinant FPV VP2 protein is immobilized on the test strip to capture FPV-specific antibodies.
2. Sample Incubation: Feline sample (serum/plasma) is applied. If present, FPV antibodies bind to the antigen.
3. Detection: A fluorescently labeled anti-feline IgG secondary antibody binds to captured FPV Ab.
4. Result Interpretation: Fluorescence intensity is measured by a handheld reader:
   • Positive: Signal above cutoff indicates protective antibody levels (typically ≥1:40 titer).
   • Negative: No significant antibodies detected.

Key Features:

• Rapid: Results in 10 minutes.
• High Sensitivity: Detects low antibody titers (1:10 dilution).
• No Cross-Reactivity: Specific to FPV (no interference from other parvovirus antibodies).

*Store at 2–30°C. For optimal results, use fresh samples and avoid repeated freeze-thaw cycles.*