1. INTENDED USE

The fPL Rapid Test is a fluorescence-based immunoassay designed for the quantitative measurement of feline pancreatic lipase (fPL) in cat serum or plasma. This in-clinic diagnostic tool enables veterinarians to:

• Diagnose pancreatitis with high specificity for feline patients
• Monitor disease progression and treatment response in cats with pancreatic disorders
• Differentiate pancreatic from non-pancreatic causes of gastrointestinal signs (e.g., vomiting, anorexia)

Note: For veterinary use only. Not intended for human or canine diagnostics.

2. TEST PRINCIPLE

The test utilizes an advanced sandwich fluorescence immunoassay technology:

1. Capture Phase:
   • fPL in the sample binds to feline-specific anti-lipase antibodies immobilized on the test strip
2. Detection Phase:
   • A second antibody conjugated with fluorescent europium nanoparticles binds to the captured fPL
   • Forms a stable “antibody-antigen-antibody” sandwich complex
3. Measurement:
   • Fluorescence intensity is quantified by a dedicated reader
   • Provides quantitative results in 12-15 minutes with a detection range of 0.1-50 μg/L

Key Features:

• Feline-specific antibodies eliminate cross-reactivity with canine or human lipase
• Superior sensitivity detects subclinical pancreatitis (≥3.5 μg/L suggests pancreatitis)
• Stable fluorescent signal ensures reliable results unaffected by sample hemolysis or lipemia

For optimal performance, use fresh serum samples and follow recommended storage conditions for test components.