1. INTENDED USE

The FIV Ab Rapid Test is a fluorescence-based immunoassay designed for the qualitative detection of antibodies against Feline Immunodeficiency Virus (FIV) in feline whole blood, serum, or plasma. This test provides veterinarians with:

• Early screening for FIV infection in cats
• Differentiation between vaccinated and infected cats (when used with PCR confirmation)
• Health status evaluation for multi-cat households or shelter environments

Note: For veterinary diagnostic use only. Positive results should be confirmed by Western blot or PCR.

2. TEST PRINCIPLE

The test utilizes a lateral flow fluorescence immunoassay method:

1. Sample Application: The specimen migrates along the test strip, where FIV antibodies (if present) bind to:
   • Fluorescent conjugate: Anti-feline IgG labeled with europium nanoparticles
   • Capture antigen: Recombinant FIV p24 and gp40 proteins immobilized on the test line
2. Signal Generation:
   • Positive samples form a fluorescent “sandwich complex” at the test line
   • An internal control line validates proper test performance
3. Result Interpretation:
   • A portable fluorescence reader objectively measures signal intensity at 615 nm
   • Results are available in 8-10 minutes with >99% agreement to ELISA

Key Features:

• High Sensitivity: Detects antibodies as early as 4 weeks post-exposure
• Dual Protein Recognition: Minimizes false negatives from viral strain variations
• Stable Signal: Fluorescent nanoparticles resist hook effect at high antibody titers

For optimal results, use fresh samples (EDTA whole blood preferred) and follow recommended storage conditions for test cassettes.