1. INTENDED USE

The FHV Ag Rapid Test is a fluorescence-based immunoassay designed for the *qualitative detection of Feline Herpesvirus Type 1 (FHV-1) antigen* in feline ocular/nasal secretions or conjunctival swabs. This test assists veterinarians in:

• Rapid diagnosis of acute FHV-1 infection in cats with upper respiratory symptoms (sneezing, ocular discharge, or keratitis)
• Differentiating viral vs. bacterial conjunctivitis
• Monitoring viral shedding in multi-cat environments (shelters/catteries)

Note: For veterinary point-of-care use. Results should be interpreted alongside clinical signs.

2. TEST PRINCIPLE

The test utilizes a lateral flow fluorescence immunoassay format:

1. Sample Application: Specimen reacts with fluorescent nanoparticle-conjugated anti-FHV monoclonal antibodies in the test cassette.
2. Antigen Capture: FHV antigens form complexes with antibodies and migrate to the test line containing immobilized FHV-specific antibodies.
3. Signal Amplification: Accumulated fluorescent nanoparticles emit strong signals under UV excitation (365nm).
4. Result Interpretation: A portable fluorescence reader provides objective qualitative results (Positive/Negative) within 8 minutes with >95% sensitivity vs. PCR.

Key Features:

• Early Detection: Identifies viral antigens during active replication (3-7 days post-infection)
• No Cross-Reactivity: Specific to FHV-1 (no interference with FCV/Chlamydia)
• Semi-Quantitative: Fluorescence intensity correlates with viral load (reader outputs S/CO values)

Optimal performance requires proper sample collection from active lesions before antiviral treatment.