The Feline H.p Ag Rapid Test is a fluorescence-based immunoassay designed for the qualitative detection of Helicobacter pylori antigens in feline fecal samples. This test provides veterinarians with:

• Non-invasive diagnosis of active H. pylori infection in cats
• Monitoring tool for treatment efficacy and post-therapy follow-up
• Early screening for gastrointestinal disorders associated with H. pylori colonization

Note: For veterinary diagnostic use only. Results should be interpreted in conjunction with clinical symptoms and other diagnostic methods.

2. TEST PRINCIPLE

The test utilizes a sandwich fluorescence immunoassay format:

1. Sample Application: Fecal extract is added to the test device, where H.p antigens (if present) bind to anti-H.p monoclonal antibodies conjugated with fluorescent nanoparticles.
2. Lateral Flow: The antigen-antibody complex migrates to the test line containing immobilized anti-H.p antibodies, forming a sandwich complex.
3. Detection: A portable fluorescence reader quantifies the signal intensity at 525 nm emission wavelength, providing:
   • Qualitative result (Positive/Negative) within 10 minutes
   • Semi-quantitative index value for infection severity assessment

Key Features:

• High Sensitivity (98.7%): Detects as low as 5 ng/mL of H.p antigen
• No Cross-Reactivity: Specific to feline H.p strains (no interference with other GI pathogens)
• Room Temperature Stable: No cold chain requirement for test strips

Optimal performance requires fresh fecal samples collected within 24 hours and proper sample preparation.