1. INTENDED USE

The Feline Triple Antibody Rapid Test is a fluorescence-based immunoassay designed for the simultaneous detection and semi-quantitative measurement of specific antibodies against three major feline pathogens:

• Feline Panleukopenia Virus (FPV)
• Feline Calicivirus (FCV)
• Feline Herpesvirus (FHV)

This test assists veterinarians in:

• Evaluating vaccination efficacy by measuring antibody titers post-vaccination
• Assessing immune status in cats prior to boarding or travel
• Diagnosing suspected infections when paired with clinical symptoms
• Monitoring antibody levels in breeding colonies or shelters

Note: For veterinary diagnostic use only. Results should be interpreted in conjunction with clinical findings.

2. TEST PRINCIPLE

The test utilizes a multiplex fluorescence immunoassay technology:

1. Antigen-Coated Zones: The test strip contains three distinct zones coated with:
   • FPV VP2 recombinant antigen
   • FCV capsid protein
   • FHV glycoprotein B
2. Sample Application: Serum/plasma is mixed with fluorescent nanoparticle-conjugated anti-feline IgG.
3. Antibody-Antigen Binding: Specific antibodies in the sample bind to their respective antigens, forming immune complexes with the fluorescent conjugates.
4. Fluorescence Detection: A portable reader measures:
   • Signal intensity at each pathogen-specific zone
   • Provides semi-quantitative results (Negative/Low/Medium/High) for each antibody within 15 minutes

Key Features:

• Multiplex Detection: Simultaneous measurement of three antibodies from a single sample
• High Sensitivity: Detects antibody levels as low as 50 mIU/mL
• Differentiation Capability: Distinguishes between vaccination-induced and natural infection antibodies
• Stable Results: Fluorescence signals remain readable for 24 hours post-test

For optimal performance:

• Use fresh serum/plasma (avoid hemolyzed samples)
• Store reagents at 2-8°C prior to use
• Include positive and negative controls with each test run