1. INTENDED USE

The Canine Relaxin Rapid Test is a fluorescence-based immunoassay designed for the early detection and confirmation of pregnancy in dogs by measuring relaxin hormone levels in serum or plasma. This test enables veterinarians and breeders to:

• Confirm pregnancy as early as 20-25 days post-breeding (vs. 45+ days for ultrasound)
• Differentiate true pregnancy from pseudopregnancy
• Monitor high-risk pregnancies by tracking relaxin trends
• Support breeding management decisions

Note: For veterinary use only. Not validated for other species.

2. TEST PRINCIPLE

This test utilizes a sandwich fluorescence immunoassay methodology:

1. Capture Phase:
   • Anti-relaxin antibodies are immobilized on the test strip
   • Canine relaxin in the sample binds to these antibodies
2. Detection Phase:
   • Fluorescent nanoparticle-conjugated antibodies bind to the captured relaxin
   • Forms an antibody-relaxin-antibody “sandwich” complex
3. Measurement:
   • Fluorescence intensity is quantified by a portable reader
   • Results are available in <15 minutes with sensitivity of 0.5 ng/mL

Key Features:

• Breed-specific calibration (accounts for size variations)
• No cross-reactivity with progesterone or other hormones
• Quantitative results allow monitoring of relaxin trends
• Stable at room temperature (no cold chain required)

*Optimal performance requires serum/plasma collection during recommended gestational windows (20-30 days post-LH surge).*