1. INTENDED USE

The cProg Rapid Test is a fluorescence-based immunoassay designed for the quantitative measurement of progesterone in canine serum or plasma. This test provides veterinarians and breeders with a reliable tool to:

• Determine optimal breeding timing by tracking progesterone levels during estrus
• Monitor pregnancy progression and predict whelping dates
• Diagnose reproductive disorders (e.g., ovarian cysts, luteal insufficiency)
• Evaluate spay/neuter completeness in post-operative cases

Note: For veterinary diagnostic use only. Not for human testing.

2. TEST PRINCIPLE

The test utilizes a competitive fluorescence immunoassay methodology:

1. Sample Incubation: Canine serum/plasma is mixed with fluorescent-labeled progesterone conjugate
2. Competitive Binding: Endogenous progesterone competes with the labeled conjugate for limited antibody binding sites on the test strip
3. Signal Development: Fluorescence intensity is inversely proportional to progesterone concentration (higher progesterone = lower signal)
4. Quantitative Analysis: A dedicated reader provides precise progesterone levels (0.5-60 ng/mL) within 10 minutes

Key Features:

• Breeding-specific thresholds: Clear interpretation for LH surge (2-3 ng/mL) and ovulation (5-8 ng/mL)
• High precision: CV <8% across measurable range
• Whole-blood compatible: Eliminates serum separation requirement
• Stable reagents: 24-month shelf life at 2-8°C

For optimal results, collect samples at consistent times (morning preferred) and avoid hemolyzed specimens.