1. INTENDED USE

The Canine Parvovirus (CPV) & Coronavirus (CCV) Antigen Rapid Test is a fluorescence-based immunoassay designed for the simultaneous and differential detection of CPV and CCV antigens in canine fecal samples. This test provides veterinarians with:

• Early diagnosis of CPV (a life-threatening disease) and CCV infections
• Rapid differential detection between CPV and CCV (common causes of viral enteritis)
• Point-of-care testing capability for immediate clinical decisions
• Monitoring of disease progression and treatment efficacy

Note: For veterinary diagnostic use only. Confirmatory testing may be required for low-positive results.

2. TEST PRINCIPLE

The test utilizes a dual-channel fluorescence immunoassay technology:

1. Capture Phase:
   • Fecal sample extracts are applied to test strips containing:
     • Anti-CPV monoclonal antibodies (test line 1)
     • Anti-CCV monoclonal antibodies (test line 2)
   • Control line contains species-specific IgG
2. Detection Phase:
   • Fluorescent nanoparticle-conjugated antibodies bind to captured antigens
   • CPV and CCV antigens are detected simultaneously in separate channels
3. Result Interpretation:
   • A portable fluorescence reader quantifies signals at 650nm (CPV) and 520nm (CCV)
   • Provides semi-quantitative results within 10 minutes
   • Detection limit: ≥1.5 ng/mL for CPV; ≥2.0 ng/mL for CCV

Key Features:
✔ High sensitivity (98.2%) and specificity (99.1%) compared to conventional ELISA
✔ Dual detection eliminates cross-reactivity between CPV/CCV
✔ Stable fluorescent labels allow result documentation and re-evaluation

*Store at 2-30°C. Use fresh fecal samples (<24h) for optimal accuracy.*