1. INTENDED USE

The CGIA Ag Rapid Test is a fluorescence-based immunoassay designed for the qualitative detection of Giardia-specific antigens in canine fecal samples. This test assists veterinarians in:

• Rapid diagnosis of active Giardia infections in dogs
• Differentiating parasitic diarrhea from other gastrointestinal disorders
• Monitoring treatment efficacy and post-therapy follow-up
• Screening asymptomatic carriers in kennels or multi-dog environments

Note: For veterinary point-of-care use. Results should be interpreted in conjunction with clinical signs and other diagnostic methods.

2. TEST PRINCIPLE

The test utilizes a sandwich fluorescence immunoassay format with the following mechanism:

1. Sample Application:
   • Fecal extract is applied to the test device
   • Giardia antigens (if present) bind to anti-Giardia monoclonal antibodies conjugated with fluorescent nanoparticles
2. Lateral Flow:
   • The antigen-antibody complex migrates along the strip
   • Captured by immobilized anti-Giardia antibodies at the test line
3. Detection:
   • Fluorescence signal intensity at the test line is measured by a portable reader
   • Results are available within 8-10 minutes
   • Cut-off value: ≥5 ng/mL of Giardia-specific antigen

Key Features:

• High sensitivity (98.7%) and specificity (99.2%) compared to conventional microscopy
• No cross-reactivity with other common canine intestinal parasites
• Stable performance across various fecal consistencies (formed to diarrheic)
• Built-in control line validates test procedure integrity

Storage & Handling:

• Store test kits at 2-30°C
• Bring to room temperature before use
• Process samples immediately after collection for optimal results