1. INTENDED USE

The CDV Ag Rapid Test is a fluorescence-based immunoassay designed for the qualitative detection of Canine Distemper Virus (CDV) antigen in canine conjunctival swabs, nasal secretions, or whole blood/serum samples. This test provides veterinarians with:

• Early point-of-care diagnosis of active CDV infection
• Differential diagnosis of animals presenting with respiratory, neurological or gastrointestinal symptoms
• Monitoring of disease progression in infected dogs
• Outbreak management in kennels and shelters

Note: For veterinary diagnostic use only. Results should be interpreted in conjunction with clinical signs and other diagnostic methods.

2. TEST PRINCIPLE

The test utilizes a sandwich fluorescence immunoassay format:

1. Sample Application: The specimen is added to the sample well of the test device.
2. Antigen Capture: CDV antigens bind to anti-CDV monoclonal antibodies conjugated with fluorescent nanoparticles.
3. Complex Formation: The antigen-antibody complex migrates to the test line containing immobilized anti-CDV antibodies, forming a sandwich structure.
4. Signal Detection: A portable fluorescence reader quantifies the signal intensity at 525 nm emission wavelength, with results available in 8-10 minutes.

Key Features:

• High Sensitivity: Detects as few as 10³ TCID50/mL of CDV
• Excellent Specificity: No cross-reactivity with other common canine pathogens (CPV, CAV, CIV)
• Stable Results: Fluorescent signal remains stable for up to 60 minutes post-test
• Room Temperature Storage: No cold chain requirements for test strips

For optimal performance:

• Use fresh samples whenever possible
• Process swab samples within 2 hours of collection
• Follow manufacturer’s instructions for proper sample handling and test procedure