1. INTENDED USE

The Canine Cystatin C Rapid Test is a fluorescence-based immunoassay designed for the quantitative determination of Cystatin C in canine serum, plasma, or urine samples. This test provides veterinarians with an essential tool for:

• Early detection of renal dysfunction (superior to creatinine for identifying early-stage kidney disease)
• Monitoring renal disease progression and treatment efficacy
• Differentiating prerenal azotemia from intrinsic renal failure
• Assessing glomerular filtration rate (GFR) as Cystatin C is a sensitive endogenous biomarker

Note: For veterinary diagnostic use only in canine patients.

2. TEST PRINCIPLE

The test utilizes an advanced sandwich fluorescence immunoassay technology:

1. Capture Phase: Canine Cystatin C in the sample binds to specific anti-Cystatin C antibodies immobilized on the test strip.
2. Signal Generation: A second antibody conjugated with fluorescent europium nanoparticles binds to the captured Cystatin C, forming an antibody-antigen-antibody complex.
3. Quantitative Measurement: The fluorescence intensity is measured by a dedicated reader, with signal intensity directly proportional to Cystatin C concentration.

Key Features:

• Exceptional Sensitivity: Detects as low as 0.05 mg/L, enabling early renal impairment detection
• High Precision: CV <5% across the measurable range (0.1-10 mg/L)
• Rapid Turnaround: Results in 10 minutes with minimal sample preparation
• Species-Specific: Antibodies specifically developed for canine Cystatin C
• Stable Fluorescent Signal: Time-resolved fluorescence technology minimizes background interference

For optimal results, use fresh samples and follow the recommended storage conditions for reagents. Store test kits at 2-8°C and protect from light.