1. INTENDED USE

The cNT-proBNP Rapid Test is a fluorescence-based immunoassay designed for the quantitative measurement of N-terminal pro-B-type natriuretic peptide (NT-proBNP) in canine serum, plasma, or whole blood. This test provides veterinarians with a reliable cardiac biomarker for:

• Diagnosing congestive heart failure (CHF) in dogs
• Differentiating cardiac vs. respiratory causes of dyspnea or cough
• Monitoring disease progression and response to cardiac therapy
• Assessing prognosis in patients with mitral valve disease or dilated cardiomyopathy

Note: For veterinary use only. Results should be interpreted in conjunction with clinical signs and other diagnostic tests.

2. TEST PRINCIPLE

The test utilizes a sandwich fluorescence immunoassay methodology:

1. Capture Phase:
   • The sample is applied to a test strip containing immobilized anti-cNT-proBNP antibodies
   • cNT-proBNP in the sample binds to these capture antibodies
2. Detection Phase:
   • Fluorescent nanoparticle-conjugated detection antibodies bind to the captured cNT-proBNP
   • This forms a “sandwich” immune complex
3. Measurement:
   • A portable fluorescence reader quantifies the signal intensity
   • The signal is directly proportional to cNT-proBNP concentration in the sample
   • Results are available in 10-15 minutes with a detection range of 100-5000 pmol/L

Key Features:

• High sensitivity: Detects early cardiac stress before clinical signs appear
• Excellent correlation with reference laboratory methods
• Stable at room temperature – no cold chain required for test strips
• Minimal sample volume (50 μL) required

For optimal results, follow manufacturer’s instructions regarding sample handling and test procedure.