INTENDED USE

Sickle cell disease is an inherited blood disorder.It is marked by flawed hemoglobin. That’s the protein in red blood cells that carries oxygen to the tissues of the body. So, sickle cell disease interferes with the delivery of oxygen to the tissues. SCD is a genetic condition that is present at birth. It is inherited when a child receives two genes-one from each parent that code for abnormal hemoglobin.

Normal red blood cells can live up to 120 days.But,sickle cells only live for about 10 to 20 days.The sickled cells also damage the spleen.This puts you are at greater risk for infections.
There are several types of SCD.The specific type of SCD a person has depends on the genes they inherited from their parents.People with SCD inherit genes that contain instructions,or code,for abnormal hemoglobin.
People who have this form of SCD inherit two genes, one from each parent,that code for hemoglobin”S.”Hemoglobin S is an abnormal form of hemoglobin that causes the red cells to become rigid,and sickle shaped.This is commonly called sickle cell anemia and is usually the most severe form of the disease.
People who have this form of SCD inherit a hemoglobin “S” gene from one parent and a gene for a different type of abnormal hemoglobin called”C”from the other parent.This is usually a milder form of SCD.
People who have sickle cell trait(SCT) inherit a hemoglobin”S”gene from one parent and a normal gene (one that codes for hemoglobin”A”) from the other parent.People with SCT usually do not have any of the signs of the disease.However,in rare cases, a person with SCT may develop health problems. Additionally,people who have SCT can pass the abnormal hemoglobin “S” gene on to their children.

Pinciple

Sickle Screen Rapid test is a double antibody lateral flow chromato- graphic immunoassay.The test cassette consists of 1)a nitrocellulose membrane strip containing a test line (HA, HS and HC) pre-coated with monoclonal antibodies and a control line (C line) pre-coated with Goat anti Mouse IgG.2) Conjugate pad containing monoclonal antibodies to sickle cell conjugated with colloidal gold.When an adequate volume of test specimen applied to the sample well of the test cassette,the specimen migrates by capillary action across the cassette.Hemoglobin variant A,S and C,if present in the specimen, will bind to the antibody and will form the complex with coated and conjugated antibodies.The immune-complex migrates on the membrane,when it reaches the test line; it will be captured by pre-coated antibodies and forms a visible Pink / Purple band. The control line is a procedural control should appear always.