INTENDED USE

Pregnancy diagnosis is an important part in reproduction management of ruminants. In the 1970s, many studies related to cattle pregnancy were conducted, and various substances were discovered and identified. A polymorphic family of placenta-expressed proteins has been discovered in ruminant species and used for pregnancy diagnosis in the last years.

Among them, the pregnancy-associated glycoproteins (PAG) are synthesized in the mono- and bi-nucleate cells of the ruminant’s trophectoderm and is secreted from the conceptus between days 20 and 28 as the extra embryonic trophoblastic cell layer relocates to the endometrium, enabling successful implantation and continuation of pregnancy in ruminants.
The PAG is widely recognized as one of the most efficient markers for detecting pregnancy in cattle.

Pinciple

Bovine Pregnancy Test Kit is a lateral flow chromatographic immunoassay that detects early PAG contained in bovine whole blood, serum, and plasma.
If PAG is present in the sample, it binds to the PAG-specific antibody– nanoparticle conjugate. A red line appears at the location of the test line due to binding to the PAG-specific antibody that moves through the membrane by capillary action and is dispensed on the membrane. If PAG is not present in the sample, no reaction occurs on the test line and a red line appears only on the control line.
The highly selective and sensitive two monoclonal antibodies to PAG are used as capture and detector in the kit. This kit detects PAG quickly and simply within 20 minutes after applying the sample. The RIDXTM Bovine Pregnancy Test Kit is a diagnostic reagent for pregnancy of cattle with high accuracy.